The live years investigations covered podzolic soil of agricultural sector of the mesotrophic Piaseczno Lake basin and agricultural sector black soil of the eutrophic Glębokie Lake (the Łęczyńsko-Włodawskie Lake District). The soil samples (from 0-20 cm depth) were gathered four times over the vegetative periods each year at various distances off the water surface (1^st test site – 60 m Piaseczno L. and 40 m Głębokie L.; 2^nd test site – 30 m for both lakes; 3^rd test site – bank) and urease and acid phosphatase activity was determined for them. It was found that activity of the enzymes studied demonstrated a considerable connection with these soils properties The enzymatic activity of podzolic soil revealed a clearly higher level as against black soil. The greatest difference was recorded in the bank zone and it decreased with increasing distance of soil from the lake. The urease and phosphatase activity showed annual and seasonal fluctuations and their course depended on enzyme type, soil properties and its distance off a lake.