The pot experiment was conducted in two series: without liming (0 Ca) and limed (+ Ca) on 6 treatments: 0 – soil without fertilizers, NPK – soil fertilized with nitrogen, phosphorus and potassium, NPK+S1 s.a. – soil fertilized with nitrogen, phosphorus, potassium and sulphur supplied as ammo-nium sulphate, NPK + S1 o. – soil fertilized with nitrogen, phosphorus, potassium and sulphur supplied with the waste from magnesium sulphate production and NPK + S3 s.a. – soil fertilized with nitrogen, phosphorus, potassium and sulphur supplied as ammonium sulphate in a dose three times bigger than introduced to the soil on NPK + S1 s.a. and NPK + S1 o. treatments. Spring wheat was cultivated in each year of the experiment. The sulphur content in the prepared experimental material was assessed by means of ICP-AES method on JY 238 Ultrace apparatus. An average (for three years) total yield of spring wheat (grains, straw and roots) at comparable values of standard error of arithmetic mean for individual treatments was the highest after sulphur application in the form of ammonium sulphate. In comparison with biomass yields from the treatments where a lower sulphur dose was used, either as ammonium sulphate or the waste from magnesium sulphate production, smaller biomass yield was obtained on the treatment where sulphur was applied in a thrice bigger dose. Weighed arithmetic mean of the sulphur content in grains, straw and roots of wheat fertilized with sulphur was significantly higher than the content assessed in wheat biomass not fertilized with this element. Increasing sulphur dose did not cause any significant differences in this element content in wheat biomass.