The objective of the study was to estimate the nutritive value and the functional traits of new lines of naked barley. The study comprised the grain of 23 lines of naked barley and two registered model cultivars – naked cv. Rastik and husked cv. Stratus. In samples of grain from a field experiment, from three consecutive harvest years, assays of the content of total proteins, crude fat and minerals were performed. Special attention was focused on the basic functional traits of the grain – the content of total dietary fibre and its fractional composition, analysing the content of the soluble and insoluble fractions in a water solution of enzymes and in an acid environment. It was demonstrated that the husked cultivar had a higher content of minerals (2.22% d.m.), total dietary fibre (TDF) (22.18% d.m.), AIDF fraction (4.75% d.m.) and SDF fraction (18.09% d.m.). The naked lines were characterised by a higher content of proteins (from 10.15 to 12.05% d.m.), fat (from 1.55 to 1.94% d.m.), and by a more favourable fraction composition of dietary fibre. Compared to the grain of husked barley, a significantly higher content of WSDF (from 5.51 to 7.86% d.m.) and (1-3)(1-4)-β-D-glucans (from 5.51 to 7.86% d.m.) was demonstrated. Lines STH 4561, STH 4671 and STH 4676 proved to be especially applicable for the production of functional food; they are characterised by a high content of the desirable chemical components and a high stability of the traits studied over the whole period of the experiment. Therefore they are an excellent source of genes for further breeding work.