The problem of taking up and accumulating heavy metals by plants from sewage sludge has been widely discussed in literature. However, little attention has been paid so far to the question of sewage sludge effect on macroelement content in plants and soil. Therefore the investigations were conducted to determine the influence of sewage sludge of various origins on the content and uptake of calcium, magnesium and sodium by maize and white mustard. Sewage sludge was applied at two dates – in autumn and in spring. Obtained results prove that sewage sludge applied for treatment of crops significantly increased their calcium concentrations. Larger amounts of this element accumulated in the aboveground plant parts in the autumn series. An apparent diversification has been registered in magnesium content in plant biomass of individual dates, however it is difficult to state unanimously which date of fertilization is better considering this element plant concentrations. Irrespective of the plant part, the greatest amounts of sodium were assessed in plant biomass from farmyard manure and tannery sewage treatments. Larger quantities of this element supplied with the fertilizer increased its content in plant biomass.