The influence of different doses of nitrogen applied in waste activated sludge and mineral fertilisers on the content and uptake of heavy metals by willow (Salix viminalis) was investigated. In the field experiment the willow was cultivated on objects fertilised with waste activated sludge which contained nitrogen in the following doses in kg ha-1: 100, 150 and 200. The study included also a treatment fertilised with urea at 150 kg N ha-1, phosphorus and potassium, at such doses that the ratio N:P:K was equal 1:0.35:1. The content of determined heavy metals in willow branches cultivated on the objects fertilised with waste activated sludge was the highest compared with treatments with the highest doses of nitrogen. Only the content of nickel in investigated biomass of willow stems was the highest when harvested from the treatment with 150 kg N•ha-1. The content of determined heavy metals in the biomass of willow branches fertilised with urea was similar to the content of those heavy metals in willow branches harvested from the objects fertilised with the highest doses of nitrogen in waste activated sludge. The uptake of heavy metals by the biomass of willow usually was the highest from objects fertilised with the highest doses of waste activated sludge. The highest amount of heavy metals uptake by the biomass of willow was recorded in the second year of cultivation and the lowest in the first year of cultivation.