This paper presents the preliminary evaluation of the influence of various doses of NPK fertilisers on the content of chlorophyll a, b and a+b in the leaves of two varieties of amaranth, Rawa and Aztek. In field experiments, amaranth was grown at wide row spacing on good wheat complex soil in south-eastern Poland. The following macro-element doses were applied: I – 50 kg N ha-1, 40 kg P ha-1, 40 kg K ha-1, II – 70 kg N ha-1, 50 kg P ha-1, 50 kg K ha-1, III – 90 kg N ha-1, 60 kg P ha-1, 60 kg K ha-1 and IV – 130 kg N ha-1, 70 kg P ha-1, 70 kg K ha-1. Assessments were made of chlorophyll a, b and a+b content in fresh leaf tissue during the full bloom and seed formation stages. The highest chlorophyll a and a+b content in the Rawa variety was found with dose III of NPK fertiliser, while for Aztek it was highest with dose II of NPK. Chlorophyll b content was higher per unit fresh mass of Rawa leaves than in the Aztek variety. The highest chlorophyll b content per unit of fresh mass of Rawa leaves was found with level II of macro-element fertilisers, while for Aztek this value was highest with dose III of NPK. Statistically significant dependencies were found between the NPK fertiliser doses used and the amount of chlorophyll a and b in the fresh leaf tissue of both amaranth varieties.