The study was conducted to determine the impact of long term tillage systems: conventional tillage (UP) and no-tillage (SB) on the storage of total organic carbon (Corg) and different physical organic matter fractions in soil: a) particulate organic matter (POM) – particles with diameter of 2-0.05 mm (sand-sized) and b) humified fraction (HF) associated with silt and clay particles (particles with diameter <0.05 mm). The study was based on the field experiment established in the year 1975 at the Agricultural Experimental Station, SGGW, in Chylice, located on black earth (sandy loam/loamy sand). Soil samples were collected in 2009-2010 from two depths (0-10 and 10-20 cm) in spring (May) and in autumn (October). The average content of total organic carbon was grater in soil under no-tillage (SB), especially in the top layer of 0-10 cm (about 33%). In the top layer of 0-10 cm in no-tillage about 16,9% of organic carbon was present in the POM fraction, and in conventional tillage about 13,1%. Was noted that the POM fraction is more sensitive to changes determined by tillage practices than the humified fraction (HF) and total soil organic carbon (Corg).