In winter wheat (Rysa, Mobela) subjected to pre-sowing laser stimulation (1, 2 and 3 times) an increase was found in the strength and energy of germination, and in the intensity of respiration of germinating seeds, dependent on the genotype properties of the varieties and the number of laser irradiation doses. During a seven-day sprouting process of the seeds, the level of antioxidants, i.e. carotenoids, polyphenols and vitamin C, was monitored. In the seeds of both varieties, after the laser irradiation, on the seventh day of sprouting, a decrease of carotenoids and vitamin C content was found. The polyphenols content was decreased in the seeds of the Mobela variety after laser stimulation, whereas in the seed of the variety Rysa, subjecting it to radiation once or twice stimulated an increase in the content of these compounds.