The aim of this study was to assess the influence of mineral NPK, Mg and micronutrient fertilisation on the content of macroelements and root yield of a sugar beet cultivar called Khazar. The effects of three fertilisation levels were assessed. Additionally, the highest NPK dose was analysed in treatments with NPK + Mg and micronutrients (B, Cu, Zn, Mn). The increasing level of NPK fertilisation as well as the nutrition with magnesium and micronutrients caused an increase in root yield. The highest yield of roots was achieved by beet plants fertilised with the high NPK dose combined with magnesium, boron, copper and zinc. It was demonstrated that roots fertilised with the 3NPK dose and magnesium tended to accumulate more N-total. At the same time, the applied micronutrients contributed to a decline in the N-total content of dry matter in roots. The analysed fertilisation with NPK and micronutrients did not affect the content of phosphorus, potassium and calcium in either of the two beet organs. In general, beet leaves were characterised by higher concentrations of nutrients than roots.