The objective of the research was to determine the effect of various cultivation methods and nitrogen fertilization on the content of macroelements (N, P, K, Mg, Ca) in grain of winter wheat. A field experiment with cultivation of winter wheat var. Rysa was carried out in the years 2003-2004 at the Olsztyn University of Warmia and Mazury (UMW), in the Experimental Station in Tomaszkowo. The experiment was conducted with the method of split-plot randomised blocks, in 4 replications, on heavy brown soil ranked in the good wheat complex. The first experimental factor were methods of plant cultivation: 1. Control plot – without cultivation, 2. Harrowing at the tillering stage; 3. Harrowing at the tillering stage (2 – fold); 4. Protection with Aminopielik D 450 SL herbicide; 5. Harrowing (1-fold) + protection with Aminopielik D 450 SL herbicide. The second experimental factor were variants of top dressing nitrogen fertilization (135 kg N ha-1): a) control plot – without nitrogen; b) fertilization with: 70 kg N ha-1 after re-vegetation – ammonium nitrate, 65 kg N ha-1 at the full stage of shooting – pelleted carbamide; c) 70 kg N ha-1 after re-vegetation – ammonium nitrate, 25 kg N ha 1 at the beginning of shooting stage and 40 kg N ha-1 at the end of the shooting stage – pelleted carbamide; d) as in plot "c", yet with foliar application of the second dose of nitrogen (25 kg N ha-1) with carbamide concentration of 18.1% (8.33% N). The content of macroelements in grain of winter wheat was significantly differentiated between the particular experimental years, except for nitrogen. Significant changes were also observed in contents of phosphorus and magnesium as affected by the cultivation methods applied. Variants of nitrogen fertilization exerted a significant effect only on the concentrations of nitrogen and potassium.