The material for the investigations were eight spring barley cultivars: Barke, Binal, Edgar, Hanka, Jersey, Rastik, Rataj, Scarlett. For seed irradiation a semiconductor laser was used, 200mW power and wave length of 670 nm. Different doses of laser radiation were applied, and a control variant. The sowing value of seeds (germination energy and germination capacity) and seedlings morphological characteristics: roots, coleoptile and first leaf length, were estimated. Differentiated sensitivity of barley forms to laser radiation was observed. After three irradiation cycles of barley grains a significant stimulation of first leaf length was observed.