The aim of this study was to determine phytosterol degradation during near-ambient drying of rapeseeds in a BIN silo. The contents were determined for such sterols as brassicasterol, campesterol, stigmasterol, sitosterol and avenasterol. Material for analyses comprised seeds of rape cultivar Californium. After harvest, rapeseeds were dried with the near-ambient method in a thick immobile layer of 2 m. Analyses of phytosterols contents were performed immediately after drying and after 6 and 12 months of storage at a temperature of 10±2°C. Qualitative and quantitative determinations of phytosterols were conducted using gas chromatography. Recorded results showed a significant effect of drying and storage time on contents of phytosterols. Near-ambient drying of seeds in the silo resulted in a reduction of total sterol contents by 8-14%. One-year storage of seeds dried with the near-ambient method reduced the level of sterols by 15-17%.