The aim of the study was to evaluate the impact of two different levels of agricultural technology used in trials, including the level of N fertilisation, on the content of dietary fibre and β-glucans in grain of some varieties of spring barley. The research material was grain of 10 varieties of spring barley: 3 varieties for brewing (Blask, Marthe, Stratus) and 7 varieties intended for food or feed industry (Antek, Atico, KWS Olof, Nagradowicki, Rubinek, Skarb, Suweren). Two-factor field experiment (split-plot) was conducted in growing seasons in 2010-2011 at the Institute of Cultivation and Production of Malopolska Plant Growing Company, Polanowice near Krakow. The first experimental factor consisted of the varieties and the second were 2 levels of  agricultural technology: A1 (10/20/50 NPK kg h^-1) and A2 (additional N fertilization in amount of 20 kg.h^-1 and fungicides Amistar 250 EC+ Tilt Turbo used in the shooting phase doses 0.6 + 0.6 1 ha^-1 in the earing phase, as well as growth regulator Cerone 280 SL at a dose of 0.75 1 ha^-1 applied in the late phase of shooting). The compared spring barley varieties were significantly different both in the grain content of dietary fibre as well as in the β-glucans content. The brewery varieties had a lower average level of fibre and β-glucans than the varieties of barley intended for food or feed industry. The lowest average content of dietary fibre was found in grain of barley from the brewery variety Marthe. The significant correlation between the content of dietary fibre and level of β-glucans in grain of spring barley was found. Differentiation of the content of dietary fibre in barley depending of the levels of agricultural technology applied was not confirmed. Whereas, a significant impact of cultivation technology on the content of β-glucans was observed.