In the two-year pot experiment on spring rape, spring barley, white mustard, and oats, the influence of sulphur fertilisation and liming on the content of available forms of phosphorus, potassium, and magnesium in soil was evaluated. The results received indicate that in conditions of strong acidification and lack of available sulphur, the uptake of Mg, and especially P and K used in fertilisers by the plants is limited. This is reflected in the increase in the soils richness in the available forms of these elements. The effectiveness of fertilisation with P, K, and Mg increased significantly in sites fertilised with sulphur and limed.