An effect of presowing laser biostimulation on the yield and physico-chemical properties of seeds of six different maize varieties, using He-Ne laser tight, were investigated. The seeds of following maize varieties: Malta, Contessa, Milpa, Melina, Den and Helga were exposed to the laser light utilizing a disperse beam method. Research results demonstrated that an application of the presowing laser biostimulation caused the maize yield growth due to an increase in contribution coming from plants with correctly developed cobs, growth in weight of thousand seeds, shortening in vegetation period, higher plants resistance to unfavorable climatic conditions as well as increase of protein content in the seeds.