The purpose of this paper was to describe the reaction of the soil, when the glyphosate (Roundup 360 SL) was applied in control in an apple-tree orchard to it, as well as in the comparison with weed control with using of triazine herbicides (Azotop – simazine). The soil reaction was described in comparison to the enzymatic activity of the soil, based on the results of the chemical properties. The research on the typical lessivc soil (Haplic Luvisol) that are typical of the Lublin Upland was done at the Felin Research Farm 15°15'N; 22°35'E), specifically at the research orchard run by the Orchard Department of Agriculture Academy in Lublin. Fundamental influence of herbicide kind on enzymatic activity and chemical properties of soil. The degradation of the chemical properties of a soil maintained in a herbicides fallow, using triazine herbicides was evident. This degradation was accompanied by a decrease of biochemical activity. This effect was not observed when glyphosate was used. The enzymatic activity of soil maintained in herbicide fallow, with Roundup, was a few times higher than in the soil maintained in herbicide fallow by using Azotop. The results obtained indicate that the criticism against using triazine herbicides in orchard crops is justified. Therefore it would be more appropriate to substitute other methods of weed control, to using triazine herbicides.