The present paper comprises the estimation of the effect of the components which occur in purified sewage and mineral fertilizers on the content and uptake of total sulphate and organic sulphur by spring barley. The research was carried out on the base of analysis of plant material which was obtained from the pot experiment. The soil material applied in the experiment was taken from the muck-peat soil arable layer, formed on the low-bog peat and from the podzolic soil having granulometric of the light loamy sand. The purified municipal sewage used in the investigation turned out to be a good source of sulphur for the tested plant. It is confirmed by the considerable increase of content and uptake of all sulphur forms in barley from the objects irrigated with sewage in comparison with the values observed in the plants fertilized only with mineral salts. Generally it may be stated that the application of sewage in the agricultural cultivation may significantly improve their sulphur supply.