In 1995-1998 years the field experiment was carried out at the Experimental Station belonging to the Agricultural University in Lublin. In this experiment the influence of three levels of N-fertilization (50, 100 and 150 kg N/ha) on the yield of four cultivars of winter wheat (Alba, Begra, Nike and Rosa) were tested. Yield of grain, the harvest index and the weight of 1000 kernels and bulk density were evaluated. In the grain from 1998 year the content of total protein, wet gluten and gluten index were evaluated. Levels of N-fertilization did not influence the yield of grain, but the lowest level caused however decrease the weight of 1000 kernels. The highest level of grain yield was reached by cv. Rosa, but lowest weight of 1000 kernels had the cv. Alba. The highest level of N-fertilizalion influenced no significant decreasing of wet gluten content and gluten index. The cv. Begra had the highest value of gluten index.